Journal: Applied and Environmental Microbiology

Article Title: Unique Properties of the Alpha-Helical DNA-Binding Protein KfrA Encoded by the IncU Incompatibility Group Plasmid RA3 and Its Host-Dependent Role in Plasmid Maintenance

doi: 10.1128/AEM.01771-20

Figure Lengend Snippet: KfrA dimerization in vivo and in vitro . (A) Graphic presentation of the analyzed kfrA deletion mutants. The N-terminal fragment of 53 amino acids is labeled green, with the HTH motif indicated by light green, the middle alpha-helical fragment is purple, and the repetitive motifs in the C terminus are in various shades of gray. Numbers indicate amino acid residues. (B and C) Analysis of KfrA dimerization ability in the BACTH system. Double transformants of E. coli BTH101 cyaA with compatible plasmids encoding CyaA fragment T18 or T25 fused to either intact KfrA (B) or various KfrA deletion variants (C) were analyzed on indicator MacConkey plates (pictured here) with maltose as a carbon source and by β-galactosidase assays in liquid cultures. Dark (purple) streaks are indicative of interactions between the two hybrid proteins. Numbers below the images represent β-galactosidase units with SD from at least three experiments. Two shades of gray symbolize strong and weak interactions. Absence of shading indicates no interactions. Double transformants with empty BACTH vectors were used as controls. (D) Cross-linking of purified KfrA variants. His 6 -tagged KfrA and its truncated derivatives (0.1 mg ml −1 ) were incubated with increasing concentrations of glutaraldehyde (0 to 0.05%). The products were separated by SDS-PAGE on polyacrylamide gels of appropriate concentration (18%, 15%, or 12%) and stained with Coomassie brilliant blue. Arrows indicate monomers and putative dimers; brackets encompass higher order complexes. M, protein markers (Spectra multicolor protein ladders [Thermo Fisher Scientific]; low range for KfrA 1–53 and broad range for the other KfrA variants).

Article Snippet: MacConkey agar base (BD Difco) supplemented with 1% maltose was used in the bacterial adenylate cyclase-based two-hybrid system (BACTH).

Techniques: In Vivo, In Vitro, Labeling, Purification, Incubation, SDS Page, Concentration Assay, Staining

Journal: Korean Journal of Food Science and Technology

Article Title: Storage Quality of Sulhyang Strawberries as Affected by High O₂Atmosphere Packaging

doi: 10.9721/kjfst.2013.45.2.191

Figure Lengend Snippet: Fig. 6. Changes in viable cell counts of innoculated E. coli O157:H7 (A), S. Typhimurium (B), and L. monocytogenes (C) of ‘Sulhyang’ strawberry packaged under different initial gas compositions during storage at 5oC for 12 days. Each data point presents the average and standard deviation values. Error bars show the standard deviations.

Article Snippet: 병원성 미생물 및 배지 시료에 접종할 표준 병원성 미생물 균주는 한국식품연구원 미 생물 균주은행에서 분양받은 Escherichia coli O157:H7 (ATCC43895), Salmonella Typhimurium (ATCC-14028), Listeria mono- cytogenes (ATCC-19111)를 사용하였으며, 개별 균주의 분리 및 배 양을 위해 E. coli O157:H7는 sorbitol Macconkey agar (Difco Lab.), S. Typhimurium은 보조제가 0.46% 첨가된 XLT4 agar (Merck), L. monocytogenes는 보조제가 1% 첨가된 Oxford Listeria selective agar (Merck)를 사용하였다.

Techniques: Standard Deviation

Journal: FEMS Microbes

Article Title: Radiation-response in Deinococcus bacteria: characterization of the transient IrrE–DdrO heterodimer complex

doi: 10.1093/femsmc/xtag001

Figure Lengend Snippet: Bacterial two-hybrid system showing interaction of the NTD of DdrO with IrrE. D. deserti proteins or domains fused to the T18 or T25 domain of bacterial adenylate cyclase are indicated. Cultures of reporter strain E. coli BTH101, cotransformed with two plasmids, were spotted on MacConkey plates. The appearance of a red color indicates interaction between the tested proteins or domains.

Article Snippet: The next day, 5 μl of each culture were spotted on MacConkey plates (Difco TM MacConkey Agar Base, reference number: 281810) supplemented with ampicillin, kanamycin, and 1% lactose.

Techniques: